Downstream Industrial Biotechnology: Recovery and by Michael C. Flickinger

By Michael C. Flickinger

An reasonable, simply available table reference on biomanufacturing, taken with downstream restoration and purification

Advances within the primary wisdom surrounding biotechnology, novel fabrics, and complicated engineering methods remain translated into bioprocesses that convey new items to industry at a considerably quicker velocity than such a lot different industries. commercial scale biotechnology and new production tools are revolutionizing drugs, environmental tracking and remediation, shopper items, nutrition construction, agriculture, and forestry, and stay a huge zone of research.

The downstream level in commercial biotechnology refers to restoration, isolation, and purification of the microbial items from cellphone particles, processing medium and contaminating biomolecules from the upstream approach right into a comprehensive product akin to biopharmaceuticals and vaccines.

Downstream approach layout has the best effect on total biomanufacturing rate simply because not just does the biochemistry of alternative items ( e.g., peptides, proteins, hormones, antibiotics, and complicated antigens) dictate varied tools for the isolation and purification of those items, yet contaminating byproducts may also decrease total strategy yield, and will have severe effects on scientific defense and efficacy. hence downstream separation scientists and engineers are consistently trying to dispose of, or mix, unit operations to reduce the variety of technique steps with a view to maximize product restoration at a targeted focus and purity.

Based on Wiley's Encyclopedia of business Biotechnology: Bioprocess, Bioseparation, and phone Technology, this quantity positive aspects fifty articles that supply details on down- flow restoration of cells and protein catch; procedure improvement and facility layout; gear; PAT in downstream approaches; downstream cGMP operations; and regulatory compliance.

It covers:

  • Cell wall disruption and lysis
  • Cell restoration via centrifugation and filtration
  • Large-scale protein chromatography
  • Scale down of biopharmaceutical purification operations
  • Lipopolysaccharide removal
  • Porous media in biotechnology
  • Equipment utilized in commercial protein purification
  • Affinity chromatography
  • Antibody purification, monoclonal and polyclonal
  • Protein aggregation, precipitation and crystallization
  • Freeze-drying of biopharmaceuticals
  • Biopharmaceutical facility layout and validation
  • Pharmaceutical bioburden testing
  • Regulatory requirements

Ideal for graduate and complex undergraduate classes on biomanufacturing, biochemical engineering, biophar- maceutical facility layout, biochemistry, commercial microbiology, gene expression expertise, and mobile tradition know-how, Downstream business Biotechnology is usually a hugely instructed source for execs and libraries.

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